Anti-Bacterial Potential Of Bidaraupas ( Decalobanthus mammosus) Extract Against Liver Macrophage Cells Indicated By Tuberculosis

Rachmaniyah Rachmaniyah; Win Darmanto; Dwi Winarni; Khambali Khambali

doi:10.35910/icohpj.v5i0.1044

Rachmaniyah Rachmaniyah Science And Technology Department Airlangga University
Win Darmanto Science And Technology Department Airlangga University
Dwi Winarni Science And Technology Department Airlangga University
Khambali Khambali Department of Environmental Health, Health Polytechnic, Ministry of Health, Surabaya

DOI: https://doi.org/10.35910/icohpj.v5i0.1044

Keywords: Tuberculosis, Merremia mammosa, Liver Macrophages, Mycobacterium tuberculosis, Drug Resistance

Abstract

Background: Tuberculosis (TB) is a serious infection caused by Mycobacterium tuberculosis, mainly affecting the lungs and spreading through the air. Every year, around 10 million new TB cases are reported worldwide, with Indonesia ranking third in the highest number of TB cases. TB treatment faces challenges due to drug resistance, such as MDR-TB and XDR-TB, which is driving research on alternative therapies, one of which is Merremia mammosa extract, which is known for its antibacterial and immunomodulatory potential. This study aimed to evaluate the potential of Merremia mammosa extract in enhancing the immune response, especially through liver macrophages, in mice infected with Mycobacterium tuberculosis.

Method: Mice were divided into treatment and control groups, receiving the extract at varying doses after TB bacterial injection.

Results: The results showed that most of the groups with lower necrotic cell counts came from the group with low doses, such as P11, P12, P13, P14, and P15. However, groups such as P45, which exhibit a high number of necrotic cells, may suggest that higher doses of Merremia mammosa extract may trigger an excessive inflammatory response.

Conclusion: Low-dose administration of Merremia mammosa extract is effective in reducing liver cell necrosis due to Mycobacterium tuberculosis infection. The low-dose group showed better tissue protection than high-doses, which in turn has the potential to trigger excessive inflammation.